API

The Mist data can be queried programatically. See our API Page

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MIST Version 5.0 - April 2020

Column Information

1. MasterNetID -- our own unique interaction ID
2. TaxID_A -- taxonomy id for interacting partner A, for example, 9606 for human genes
3. GeneA -- entrez geneid for interacting partner A
4. TaxID_B -- taxonomy id for interacting partner B, for example, 9606 for human genes
5. GeneB -- entrez geneid for interacting partner B
6. Rank -- confidence of interaction (high, moderate and low)
7. Interaction_type -- PPI, genetic, interolog, interolog-genetic
8. Exp_Direct -- evidence codes (MI ID) for direct interaction eg. "MI:0018" for 2-Hybrid interaction
9. Exp_Indirect -- evidence code for in-direct interaction eg. "MI:0488" for psi-mi
10. TaxID_interolog -- this column is only populated for interolog and shows the taxonomy ID of the original data
11. Source_databases -- eg. BioGrid
12. Reference -- Pubmed_ID or other reference ID (eg reference from FlyBase)
13. Reference_type -- eg. "PMID"
14. Count_direct -- count of unique evidence code (MI number) as direct interaction
15. Count_indirect -- count of unique evidence code (MI number) as in-direct interaction
16. Count_paper -- count of unique reference IDs
17. Source_Interolog -- this column is only populated for interolog and shows the MasterNetID of the original data
18. Comment

All species files zipped, tab separated text.

• Protein Protein Interactions
• Interolog of Protein Protein Interactions
• Genetic Interactions
• Interolog of Genetic Interactions

By Individual Species

Protein Protein Interactions

• Protein Protein Interactions - fission_yeast
• Protein Protein Interactions - yeast
• Protein Protein Interactions - worm
• Protein Protein Interactions - fly
• Protein Protein Interactions - zebrafish
• Protein Protein Interactions - western_clawed_frog
• Protein Protein Interactions - human
• Protein Protein Interactions - mouse
• Protein Protein Interactions - rat

Interolog of Protein Protein Interactions

• Interolog of Protein Protein Interactions - fission_yeast
• Interolog of Protein Protein Interactions - yeast
• Interolog of Protein Protein Interactions - worm
• Interolog of Protein Protein Interactions - fly
• Interolog of Protein Protein Interactions - zebrafish
• Interolog of Protein Protein Interactions - western_clawed_frog
• Interolog of Protein Protein Interactions - human
• Interolog of Protein Protein Interactions - mouse
• Interolog of Protein Protein Interactions - rat

Genetic Interactions

• Genetic Interactions - fission_yeast
• Genetic Interactions - yeast
• Genetic Interactions - worm
• Genetic Interactions - fly
• Genetic Interactions - zebrafish
• Genetic Interactions - western_clawed_frog
• Genetic Interactions - human
• Genetic Interactions - mouse
• Genetic Interactions - rat

Interolog of Genetic Interactions

• Interolog of Genetic Interactions - fission_yeast
• Interolog of Genetic Interactions - yeast
• Interolog of Genetic Interactions - worm
• Interolog of Genetic Interactions - fly
• Interolog of Genetic Interactions - zebrafish
• Interolog of Genetic Interactions - western_clawed_frog
• Interolog of Genetic Interactions - human
• Interolog of Genetic Interactions - mouse
• Interolog of Genetic Interactions - rat

Linkout

The Mist output pages can be linked to programatically.

• Generally: http://fgrtools.hms.harvard.edu/MIST/MistShowData?species=[human]&gene_list=[list of genes]&filter=[none]&type=[types]
  
• Examples:
  http://fgrtools.hms.harvard.edu/MIST/MistShowData?species=human&gene_list=217&filter=low&type=ppi_interactions
• https://fgrtools.hms.harvard.edu/MIST/MistShowData?species=drosophila&gene_list=wg,indy,lz&filter=none&type=ppi_interactions,interologs
• https://fgrtools.hms.harvard.edu/MIST/MistShowData?species=drosophila&gene_list=csw,lace,Nmd3,CG2145,wisp&filter=moderate_and_low&type=interologs_genetic,phosphoCorrelation

Parameters

• species:
  • options [human, drosophila, rat, mouse, elegans, cerevisiae, pombe, zebrafish, laevis, tropicalis ]

• gene List:
  • numeric value representing the Entrez Gene Id
  • (or) Comma or space separated list of gene names

• filter: filter out scores by rank (defaults to none)
  • [none, low, moderate_and_low]

• type:
  • all species [interolog, ppi_interactions, genetic, interologs_genetic]
  • drosophila specific : [kinaseSubstrate, phosphoCorrelation, expCorrelation, phosphoCorrelation]